CULTURE OF CLOSTRIDIUM BOTULINUM TYPE C WITH CONTROLLED pH

Structure and toxicity of Clostridium botulinum type C toxin.

chromatography on a column of 9AE Sephadex A-50 in the presence of 5% of 2mercaptoethanol at OOC. No toxic activity was detected on both the chains. They were different from each other in antigenecity, amino acid composition and electric charge. Reassociation of these two components gave a molecule with iower texic act{vity (O.1-3% of the native toxin) in the delay of S-S linkage formation, whe...

Purification and crystallization of Clostridium botulinum type C toxin.

Immunogenic and Protective Potentials of Recombinant Receptor Binding Domain and a C-Terminal Fragment of Clostridium botulinum Neurotoxin Type E

Clostridium Botulinum Type E neurotoxin heavy chain consists of two domains: the translocation domain asthe N-terminal half and the binding domain as the Cterminal half (Hc). One effective way to neutralize botulinum neurotoxin is to inhibit binding of this toxin to neuromuscular synapses with antibodies against binding domain. Two synthetic genes, coding for Hc (the full length binding d...

Interaction of pH and NaCl on culture density of Clostridium botulinum 62A.

Clostridium botulinum 62A growth rates declined with decreasing pH and increasing salt levels. Lysis rates, however, were affected only by pH. Due to competition between growth and lysis rates, an accurate assessment of interactive effects was obtained only when optical density determinations were made at multiple intervals.

Growth and toxigenesis behavior of Clostridium botulinum type E in Persian sturgeon (Acipenser persicus) Caviar prepared with various preservatives

Growth behavior of Clostridium botulinum type E beluga was studied in Acipenser persicus granular caviar treated with 5% NaCl, 5%NaCl plus 0.3% boric acid and 0.4%borax and 5%NaCl plus 0.15% methyl paraben incubated at temperatures -2, 5 and 15 °C for 224 days. The initial number of inoculated bacterial spore was 5.1×104 cfu/g caviar in each treated sample. The number of bacterial spores in sam...